Table 3.
Results of Pneumocystis jirovecii genotyping at three loci in non-immunosuppressed infants developing primary Pneumocystis primary infection.
| MLST genotype | ||||
|---|---|---|---|---|
| Bilocus genotype | ||||
| Infant Code | Sample code | MtLSU alleles | CYB alleles | SOD alleles |
| E007 | E007-2a | mtLSU2, mtLSU4 | Allele mixc | SOD2 |
| E007-3 | mtLSU2, mtLSU3, mtLSU4 | Allele mixc | Allele mixc | |
| E008 | E008-1 | ...b | CYB3 | ...b |
| E017 | E017-1 | ...b | CYB1 | SOD1 |
| E029 | E029-3 | mtLSU4 | CYB2 | SOD2 |
| E032 | E032-1 | ...b | CYB1 | ...b |
| E044 | E044-1a | ...b | CYB1 | Allele mixc |
| E044-2 | mtLSU2 | CYB1 | ...b | |
| E069 | E069-1 | Allele mixc | Allele mixc | SOD1 |
| E075 | E075-1 | mtLSU2 | ...b | ...b |
| E076 | E076-1 | mtLSU2 | CYB1 | Allele mixc |
| E082 | E082-1 | ...b | CYB6 | SOD2 |
| E089 | E089-1 | mtLSU3 | CYB1 | SOD2 |
| E103 | E103-1a | mtLSU2, mtLSU4 | CYB6 | ...b |
| E103-2 | mtLSU2 | CYB1, CYB6 | SOD2 | |
| E114 | E114-1 | mtLSU4 | CYB5 | SOD1 |
| E125 | E125-1 | mtLSU3 | ...b | ...b |
| E130 | E130-1 | mtLSU3 | CYB2 | Allele mixc |
| E138 | E138-1 | mtLSU3 | CYB5 | Allele mixc |
| E143 | E143-2 | mtLSU2 | ...b | ...b |
| E154 | E154-1 | ...b | CYB1, CYB6 | ...b |
| E156 | E156-1 | mtLSU2 | CYB2 | SOD2 |
| E159 | E159-1 | mtLSU2 | ...b | ...b |
| E165 | E165-1 | mtLSU2, mtLSU3 | CYB1 | Allele mixc |
| E169 | E169-1 | mtLSU2 | CYB2 | ...b |
| E171 | E171-1 | ...b | CYB1 | ...b |
| E177 | E177-1 | mtLSU2 | CYB1 | ...b |
| E190 | E190-1 | mtLSU3 | CYB1 | ...b |
| E206 | E206-1 | mtLSU3, mtLSU4 | CYB1 | SOD2 |
The alleles were identified considering changes at nucleotide positions described elsewhere by Beard et al.23, Esteves et al.16, and Maitte et al.17.
CYB, cytochrome b gene; mtLSU, mitochondrial large subunit ribosomal RNA gene; SOD, superoxide dismutase gene.
aThe infants underwent nasopharyngeal aspirates two times, only one sample was considered for statistical analysis.
b“…”, typing failure.
cAllele mix, presence of several different alleles that were not identified.