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. 2015 Jul 10;66(20):6259–6271. doi: 10.1093/jxb/erv333

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com

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Fig. 4. — RNAi silencing of IiPLR1, IiPLR2, and IiPLR3 in I. indigotica hairy roots. (A) Schematic representation of the RNAi vector. P_35S, CaMV 35S promoter; hyg^r, hygromycin resistance gene; Intron, the Pdk intron; PLR, the 400–500bp fragment of the coding region of IiPLRs; T_35S, CaMV 35S terminator; T_ocs, terminator of the octopine synthase gene; LB, T-DNA left border; RB, T-DNA right border. Restriction sites are marked. (B) IiPLR1, IiPLR2, and IiPLR3 transcript abundance and (C) lariciresinol content in IiPLR1-RNAi, IiPLR2-RNAi, and IiPLR3-RNAi lines. (D) Phenotype of IiPLR-RNAi hairy roots before (upper) and after (lower) phloroglucinol-HCl staining.