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. 2015 Jul 10;66(20):6259–6271. doi: 10.1093/jxb/erv333

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com

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Fig. 7. — Overexpression of IiPLR1 in I. indigotica hairy roots. (A) Schematic representation of the overexpression vector. P_35S, CaMV 35S promoter; P_2×35S, CaMV 35S double promoter; hyg^r, hygromycin resistance gene; Bar, herbicide resistance gene; IiPLR1, the IiPLR1 coding sequence; polyA, poly(A) tail; T_35S, CaMV 35S terminator; T_nos, nopaline synthase terminator; LB, T-DNA left border; RB, T-DNA right border. The flag-tag and restriction sites are marked. (B) IiPLR1 transcript abundance, (C) IiPLR1 transcript expression, and (F) lariciresinol content in transgenic hairy root lines. (D) Time courses of growth of hairy root lines ovx-2, ovx-8, ovx-9, WT, and CK. (E) Phenotype of CK control (left) and IiPLR1-OVX hairy roots (right) before (upper) and after (lower) phloroglucinol-HCl staining.