Figure 1.

Infant and adult AMϕs have a similar immunophenotype. (A) Representative flow cytometry plots showing gating strategy to differentiate AMϕs based on their high autofluorescence (in the 488 530/30 FITC channel) and high CD64 expression, from lineage positive cells (CD3+ T cells, CD19+ B cells, CD56+ NK cells, CD66b+ granulocytes, and CD235a+ erythrocytes) and monocytes (low autofluorescence in 488 530/30 FITC channel, lineage negative and CD64+). (B) Flow cytometry of AMϕs from seven infant BAL samples (blue representative histogram) compared with seven adults (red representative histogram), with fluorescence minus one (gray representative histogram). Line represents median. MFI, mean fluorescence intensity.