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. 2020 Mar 24;11:486. doi: 10.3389/fimmu.2020.00486

Figure 2.

Figure 2

Impaired control of Mtb by Infant AMϕs. (A) Phagocytosis of Mtb-LuxG13 by AMϕs from seven infants and seven adults after 2 h of incubation at MOI 5:1. Proportion of phagocytosed bacteria assessed by measurement of autoluminescence after removal of extracellular bacilli with PBS washes, as a percentage of autoluminescence before PBS washes. Each data point represents a single observation from a single participant. Bar denotes median; (B) Ability of AMϕs to restrict replication of Mtb-LuxG13. Data points represent mean (bar = SD) of AMϕs from seven infants (empty circles) compared with seven adults (filled circles), and line shows fold-change in autoluminescence calculated from serial measurements relative to zero time point, following 2 h of incubation with Mtb-LuxG13 at MOI 5:1. Significance determined by 2-way ANOVA and denoted by ****(p < 0.0001); (C) Soluble inflammatory mediator production in culture supernatants by infant vs. adult AMϕs at 24 h post infection with Mtb-LuxG13. Significance determined by Mann-Whitney test. Statistically significant results denoted by **(q < 0.01) and after correction for multiple comparisons by Benjamini-Hochberg method.