Skip to main content
. 2020 Mar 24;11:486. doi: 10.3389/fimmu.2020.00486

Figure 4.

Figure 4

Polarized gene expression affecting specific functional pathways in Mtb-infected AMϕs. (A) Expression profile of SDE genes in Mtb-stimulated infant vs. adult AMϕs involved in lysosome function (GO: 0000323), response to bacterium (GO:0009617) and cellular response to IFNγ (GO: 0071346); and (B) Expression profile of all SDE genes encoding chemokines in Mtb-stimulated infant vs. adult AMϕs. Scale intensity represents Z-score. (C) Enrichment of 36 genes in the transcriptome of Mtb-stimulated infant/adult AMϕs previously shown to be upregulated in AMϕ in response to H37Rv Mtb relative to the avirulent H37Ra Mtb strain in (27); and (D) Enrichment of 12 genes in the transcriptome of Mtb-stimulated infant/adult AMϕs previously shown to be upregulated in Mtb-stimulated monocyte-derived macrophages from patients who had previously recovered from TB meningitis and pulmonary TB, relative to equivalents from patients with latent TB (28). GSEA plots showing all genes from Mtb-stimulated dataset ranked horizontally from highest differential expression in infants (red) to highest differential expression in adults (blue). The enrichment profile (green line) shows the degree of overrepresentation of previously published sets of genes (vertical black lines). Analysis performed using GSEA software (Version 6.3, Broad Institute).