Skip to main content
. 2020 Feb 24;43(5):1375–1386. doi: 10.3892/or.2020.7515

Figure 2.

Figure 2.

METTL14 interference and overexpression affect m6A levels in BC cell lines. (A and B) Overexpression and interference efficiency of METTL14 were determined in MDA-MB-231 and MCF-7 cells by RT-qPCR. The effect of METTL14 overexpression (C and E) and knockdown (D and F) on the m6A level was measured using the m6A RNA Methylation Quantification Kit in MDA-MB-231 and MCF-7 cells. The results are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 and ****P<0.0001. METTL14, methyltransferase-like 14; BC, breast cancer; m6A, N6-methyladenosine. MCF-7 pc-METTL14 and MDA-MB-231 pc-METTL14, METTL14-overexpressing cell lines; MCF-7 si-METTL14 and MDA-MB-231 si-METTL14, METTL14-knockdown cell lines; RT-qPCR, reverse transcription-quantitative real-time PCR.