Table I.
Primary and secondary antibodies used in the WB analysis.
| Antibody | Dilution | Catalog no. | Supplier |
|---|---|---|---|
| WIPI2 | 1:1,000 | ab105459 | Abcam |
| GAPDH | 1:1,000 | 5174 | Cell Signaling Technology |
| β-actin | 1:1,000 | 3700 | Cell Signaling Technology |
| AMPK | 1:1,000 | 5831 | Cell Signaling Technology |
| p-AMPK | 1:1,000 | 50081 | Cell Signaling Technology |
| AKT | 1:1,000 | 4691 | Cell Signaling Technology |
| p-AKT | 1:1,000 | 5012 | Cell Signaling Technology |
| Caspase 3 | 1:1,000 | 9662 | Abcam |
| Bcl2 | 1:1,000 | ab32124 | Abcam |
| Bax | 1:1,000 | ab32503 | Abcam |
| Cyclin D1 | 1:8,000 | ab134175 | Abcam |
| Goat anti-mouse IgG H&L (HRP) | 1:10,000 | ab205719 | Abcam |
| Goat anti-rabbit IgG H&L (HRP) | 1:10,000 | ab205718 | Abcam |
WB, western blot; WIPI2, WD-repeat domain phosphoinositide-interacting protein 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; AMPK, AMP-activated protein kinase; AKT, protein kinase B; Bcl2, B-cell lymphoma 2; Bax, Bcl-2-associated X protein; p-, phosphorylated.