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. 2004 Jan 1;50(1):67–72. doi: 10.1373/clinchem.2003.023663

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© 2004 The American Association for Clinical Chemistry

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Figure 1. — Real-time quantitative RT-PCR assays for SARS CoV.

(A), calibration curve for quantitative analysis of ORF 1b gene of SARS CoV. (B), calibration curve for quantitative analysis of N gene of SARS CoV. The Ct is the number of PCR cycles required for the fluorescence intensity of the reaction to reach a predefined threshold. The Ct is inversely proportional to the logarithm of the starting concentration of plasmid DNA. Equations for the regression lines and correlation coefficients are indicated.