Table 1.
Code | Strain | RNA, copies/mL1 | Laboratory | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 22 | 3 | 4 | 5 | 62 | 7 | 82 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | Total | |||||||||||||||||||||||||||||||||
2 | WNV-NY99 | 1.8E7 | +3 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | – | 29 | ||||||||||||||||||||||||||||||
5 | WNV-PaAn001 | 5.0E6 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | – | + | + | + | + | + | – | + | + | + | + | + | + | – | – | 26 | ||||||||||||||||||||||||||||||
9 | WNV-NY99 | 1.8E6 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | – | – | + | – | 27 | ||||||||||||||||||||||||||||||
1 | WNV-Ug37 | 1.4E6 | + | + | + | + | + | + | + | + | + | – | – | – | – | – | – | + | – | + | – | – | – | – | – | – | – | – | – | + | + | – | 13 | ||||||||||||||||||||||||||||||
12 | WNV-NY99 | 1.8E5 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | – | – | – | – | – | – | – | – | 22 | ||||||||||||||||||||||||||||||
4 | WNV-NY99 | 1.8E4 | + | + | + | + | + | + | + | + | – | + | + | + | + | + | + | + | + | – | + | + | – | + | – | – | – | – | – | – | – | – | 19 | ||||||||||||||||||||||||||||||
6 | WNV-Kunjin | 2.6E3 | + | – | + | + | + | – | + | + | + | + | – | – | – | – | – | – | – | + | – | + | – | + | – | – | – | – | – | – | – | – | 11 | ||||||||||||||||||||||||||||||
10 | SLEV/JEV4 | 2E8/2E7 | – | – | S | S | – | – | – | – | – | + | – | – | – | – | – | – | – | – | – | + | – | – | – | – | – | – | – | – | – | – | 2 | ||||||||||||||||||||||||||||||
11 | YFV/TBEV4 | 1E6/1E6 | – | – | – | Y | – | – | F | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | ||||||||||||||||||||||||||||||
3 | DenV 1–45 | 2E6–2E8 | – | – | D | D | – | – | D | D | – | – | – | – | – | – | – | – | – | D | – | – | – | – | D | – | – | – | – | + | + | – | 2 | ||||||||||||||||||||||||||||||
7 | Negative | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + | – | – | – | – | – | – | – | – | – | – | – | 1 | ||||||||||||||||||||||||||||||
8 | Negative | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Confirmed by real-time PCR in triplicate. Samples 2, 9, 12, and 4 constitute a dilution series: their concentrations are provided as expected upon dilution factors, but they have also been confirmed by real-time RT-PCR.
These participants used commercial RT-PCR assays (Revertal, Qiagen).
+, West Nile virus detected. Viruses other than West Nile virus detected: S, St. Louis encephalitis virus; Y, yellow fever virus; D, dengue virus; F, unspecified flavivirus.
SLEV, St. Louis encephalitis virus; JEV, Japanese B encephalitis virus; TBEV, tick-borne encephalitis virus.
RNA copies in detail: DenV-1, 1.5E8/mL; DenV-2, 2.0E8/mL; DenV-3, 2.0E6/mL; DenV-4, 2.2E8/mL.