Table 3.
Characteristics of the different protein microarrays.
| RPPM | FPPM | |||
|---|---|---|---|---|
| Planar microarray | Bead-based array | |||
| Immobilization | Cell or tissue lysates | Peptide, protein, or antibody | Peptide, protein, or antibody | |
| Labeling of analyte | Without | With or without | With or without | |
| Quantification | Relative | Relative or absolute | Relative or absolute | |
| Number of spots | >1000 | >1000 | ≤100 | |
| Throughput | Low | Low | High | |
| Automation | Low | Low | High | |