Table 3.
Characteristics of the different protein microarrays.
RPPM | FPPM | |||
---|---|---|---|---|
Planar microarray | Bead-based array | |||
Immobilization | Cell or tissue lysates | Peptide, protein, or antibody | Peptide, protein, or antibody | |
Labeling of analyte | Without | With or without | With or without | |
Quantification | Relative | Relative or absolute | Relative or absolute | |
Number of spots | >1000 | >1000 | ≤100 | |
Throughput | Low | Low | High | |
Automation | Low | Low | High |