Table 1.

Primers and probes used in real-time PCR assay for HBoV

Gene	Primer–probe	Sequence (5′–3′)	Positiona
NS1	Forward	TGA ACC TGA AGA GGC TGA CAT ATT TC	656–681
	Reverse	CAT GTT GCC GCC AGT AAC TC	718–737
	Probe	CAC CCC ATG CCT CTC G	702–717
NP-1	Forward	CAG CCA CCT ATC GTC TTG CA	2513–2532
	Reverse	CCC TCG TCT TCA TCA CTT GGT	2551–2571
	Probe	CTG CTT CGA AGA CCT C	2533–2548
VP1	Forward	AGC TGT CAC TTC TCA CCA CAA G	3679–3700
	Reverse	TTG CTT TAG GTC TGA AGC GCT TAT	3725–3748
	Probe	ATT GGC AGC GCC TTA C	3701–3716

^aNucleotide position was designated according to HBoV strain ST1 (GenBank accession no. DQ000495)