Table 2.
List of human pathogens used for real-time PCR specificity test
| Viruses and bacteriaa | Sourceb | Titer |
|---|---|---|
| RNA viruses | ||
| Influenza virus A | 2 × 107 TCID50/ml | |
| Influenza virus B | 4.2 × 105 TCID50 | |
| PIV1/2/3 | >5 × 105 TCID50/ml | |
| RSV | ATCC VR-26 | 4 × 107 TCID50/ml |
| HCoV 229E | ATCC VR-740 | 3 × 104 TCID50/ml |
| HCoV OC43 | ATCC VR-1558 | 4 × 105 TCID50/ml |
| Rhinovirus 13 | ATCC VR-1123 | 106 TCID50/ml |
| Enterovirus | 5.2 × 105 TCID50/ml | |
| DNA viruses | ||
| Parvovirus B19 | 104 copies/ml | |
| Adenovirus 11 | 5 × 105 pfu/ml | |
| HSV-2 | ATCC VR-540 | 2.5 × 104 TCID50/ml |
| HHV-6 | ATCC VR-1480 | 3 × 104 TCID50/ml |
| VZV | ATCC VR-1503 | 4.5 × 104 TCID50/ml |
| Bacteria | ||
| Haemophilus influenzae | 2 × 105 CFU | |
| Legionella pneumophila | 3 × 105 CFU | |
| Streptococcus pneumoniae | 1.5 × 105 CFU | |
| Mycoplasma pneumoniae | 3 × 106 CFU | |
| Chlamydia pneumoniae | 103 IFU/ml | |
HSV-2, herpes simplex virus 2; HHV-6, human herpesvirus 6; VZV, varicella-zoster virus; PIV1/2/3, parainfluenza virus 1/2/3; RSV, respiratory syncytial virus; HCoV, human coronavirus.
a
These bacteria were provided by Division of Bacterial Respiratory Infections, Center for Infectious Diseases, Korea National Institute of Health.
b
If not specified, strains are clinical isolates.