Table 2.
Human metapneumovirus (type A)-positive nasopharyngeal aspirate samples inoculated and propagated at 37 °C onto African green monkey kidney (AGMK), Vero, HEp-2, LLC-MK2 and human embryonic lung (HELF) cell cultures
| Spec. #, cell culture passage | Detection in cell cultures by |
|
|---|---|---|
| RT-PCR | CPE | |
| 6621, AGMK/1 | Positive | 2+ |
| 6621, AGMK/2 | Positive | 2+ |
| 6621, AGMK/5 | Positive | 3+ |
| 6621, AGMK/9 | Positive | 4+ (syncytia and single refractile cells) |
| 6621, AGMK/2-HELF/1 | Positive | 0 |
| 6621, AGMK/2-HELF/2 | Positive | 0 |
| 6621, AGMK/2-HELF/3 | Negative | 0 |
| 6621, AGMK/2-LLC-MK2/1 | Positive | 0 |
| 6621, AGMK/2-LLC-MK2/2 | Positive | 0 |
| 6621, AGMK/2-LLC-MK2/5 | Positive | 1+ |
| 6621, AGMK/2-LLC-MK2/8 | Positive | 4+ (generalized clumps of granular dull cells) |
| 6621, Vero/1 | Positive | 0 |
| 6621, Vero/2 | Positive | 0 |
| 6621, Vero/3 | Negative | 0 |
| 3636, AGMK/1 | Positive | 1+ |
| 3636, AGMK/2 | Positive | 2+ |
| 3636, AGMK/5 | Positive | 3+ |
| 3636, AGMK/3-HELF/1 | Positive | 0 |
| 3636, AGMK/3-HELF/2 | Negative | 0 |
| 3636, AGMK/5-LLC-MK2/1 | Positive | 1+ |
| 3636, AGMK/5-LLC-MK2/2 | Positive | 2+ |
| 3636, AGMK/5-LLC-MK2/5 | Positive | 3+ |
| 3636, Vero/1 | Positive | 0 |
| 3636, Vero/2 | Negative | 0 |
| 3636, HEp-2/1 | Positive | 0 |
| 3636, HEp-2/2 | Negative | 0 |
Virus growth as detected by RT-PCR and cytopathic effect (CPE).