Table 1.
Primers and probes.
| Virus | Sequence 5′–3′ | Target gene | Labels 5′/3′ | |
|---|---|---|---|---|
| Influenza A | F | GACAAGACCAATCCTGTCACYTCTG | ||
| R | AAGCGTCTACGCTGCAGTCC | M | ||
| P | TTCACGCTCACCGTGCCCAGTGAGC | LCRED610/BBQ | ||
| Influenza B | F | TCGCTGTTTGGAGACACAAT | ||
| R | TTCTTTCCCACCGAACCA | M | ||
| P | AGAAGATGGAGAAGGCAAAGCAGAACT | CYAN500/DB | ||
| Enterovirus | F | GGCCCTGAATGCGGCTAAT | ||
| R | GGGATTGTCACCATAAGCAGCC | 5′-UTR | ||
| P | GCGGAACCGACTACTTTGGGT | FAM/MGB-NFQ | ||
| Adenovirus | F | CAGGACGCCTCGGRGTAYCTSAG | ||
| R | GGAGCCACVGTGGGRTT | Hexon | LCRED670/BBQ | |
| P | CGGGTCTGGTGCAGTTTGCCCGC | |||
| RSV | F | ATGAACAGTTTAACATTACCAAGT | ||
| R | GTTTTGCCATAGCATGACAC | F | ||
| P1 | TGACTTCAAAAACAGATGTAAGCAGCTCC | LCRED610/BBQ | ||
| P2 | TTATGACATCAAAAACAGACATAAGCAGCTCAG | |||
| Rhinovirus | F | CAAGCACTTCTGTTTCCCC | ||
| R | GGCAGCCACGCAGGC | 5′-UTR | ||
| P1 | TAGACCTGGCAGATGA+G+G+CT | FAM/BBQ | ||
| P2 | TAGTTTGGTCGA+T+GA+GGCT | FAM/BBQ | ||
| P3 | CTAGTYTGGTCGAT+G+A+GGC | FAM/BBQ | ||
| Coronavirus | F1 | GGTGGYTGGGAYGATATGTTACG | ||
| F2 | GCTRAGCATGATTTCTTTACTTGG | Replicase | ||
| R1 | KRTTTGGCATAGCACGATCACA | |||
| R2 | CARTYTTKTTCATCAAAGTTACGCA | Replicase | ||
| P1 | ATGTTGACAAYCCTGTWCTTATGGGTTGGG | FAM/MGB-NFQ | ||
| P2 | CAGARTCATTTATGGTAATGTTAGTAGACA | FAM/MGB-NFQ | ||
| Metapneumovirus | F | AGCTTCAGTCAATTCAACAGAAG | ||
| R | CCTGCAGATGTYGGCATGT | F | ||
| P | TGTTGTGCGGCAGTTTTCAGACAATGC | LCRED670/BBQ | ||
| Parechovirus | F | CTGGGGCCAAAAGCCA | ||
| R | GGTACCTTCTGGGCATCCTTC | 5′-UTR | ||
| P | AAACACTAGTTGTAWGGCCC | FAM/MGB-NFQ | ||
| Parainfluenzavirus 1 | F | ATCTCATTATTACCYGGACCAAGTCTACT | ||
| R | CATCCTTGAGTGATTAAGTTTGATGAATA | HN | ||
| P | AGGATGTGTTAGAYTACCTTCATTATCAATTGGTGATG | CYAN500/DB | ||
| Parainfluenzavirus 2 | F | CTGCAGCTATGAGTAATC | ||
| R | TGATCGAGCATCTGGAAT | NP | ||
| P | AGCCATGCATTCACCAGAAGCCAGC | LCRED610/BBQ | ||
| Parainfluenzavirus 3 | F | ACTCTATCYACTCTCAGACC | ||
| R | TGGGATCTCTGAGGATAC | NP | ||
| P | AAGGGACCACGCGCTCCTTTCATC | LCRED670/BBQ | ||
| Parainfluenzavirus 4 | F | GATCCACAGCAAAGATTCAC | ||
| R | GCCTGTAAGGAAAGCAGAGA | NP | ||
| P | TATCATCATCTGCCAAATCGGCAA | HEX/BHQ | ||
| HBoV | F | CAAATCTCTTCTGGCTACACG | ||
| R | CTCTGCGATCTCTATATTGAAGG | NS1 | ||
| P | ATGTTGCCGCCAGTAACTCCACC | LCRED670/BBQ | ||
| IC (EAV) | F | CATCTCTTGCTTTGCTCCTTAG | ||
| R | AGCCGCACCTTCACATTG | Replicase | ||
| P | TGTGGGCAATAATGTTGTTCTGACAGCG | HEX/BHQ |
+ denotes a locked nucleic acid (LNA) nucleotide.