Table 1.
Primers and probes.
Virus | Sequence 5′–3′ | Target gene | Labels 5′/3′ | |
---|---|---|---|---|
Influenza A | F | GACAAGACCAATCCTGTCACYTCTG | ||
R | AAGCGTCTACGCTGCAGTCC | M | ||
P | TTCACGCTCACCGTGCCCAGTGAGC | LCRED610/BBQ | ||
Influenza B | F | TCGCTGTTTGGAGACACAAT | ||
R | TTCTTTCCCACCGAACCA | M | ||
P | AGAAGATGGAGAAGGCAAAGCAGAACT | CYAN500/DB | ||
Enterovirus | F | GGCCCTGAATGCGGCTAAT | ||
R | GGGATTGTCACCATAAGCAGCC | 5′-UTR | ||
P | GCGGAACCGACTACTTTGGGT | FAM/MGB-NFQ | ||
Adenovirus | F | CAGGACGCCTCGGRGTAYCTSAG | ||
R | GGAGCCACVGTGGGRTT | Hexon | LCRED670/BBQ | |
P | CGGGTCTGGTGCAGTTTGCCCGC | |||
RSV | F | ATGAACAGTTTAACATTACCAAGT | ||
R | GTTTTGCCATAGCATGACAC | F | ||
P1 | TGACTTCAAAAACAGATGTAAGCAGCTCC | LCRED610/BBQ | ||
P2 | TTATGACATCAAAAACAGACATAAGCAGCTCAG | |||
Rhinovirus | F | CAAGCACTTCTGTTTCCCC | ||
R | GGCAGCCACGCAGGC | 5′-UTR | ||
P1 | TAGACCTGGCAGATGA+G+G+CT | FAM/BBQ | ||
P2 | TAGTTTGGTCGA+T+GA+GGCT | FAM/BBQ | ||
P3 | CTAGTYTGGTCGAT+G+A+GGC | FAM/BBQ | ||
Coronavirus | F1 | GGTGGYTGGGAYGATATGTTACG | ||
F2 | GCTRAGCATGATTTCTTTACTTGG | Replicase | ||
R1 | KRTTTGGCATAGCACGATCACA | |||
R2 | CARTYTTKTTCATCAAAGTTACGCA | Replicase | ||
P1 | ATGTTGACAAYCCTGTWCTTATGGGTTGGG | FAM/MGB-NFQ | ||
P2 | CAGARTCATTTATGGTAATGTTAGTAGACA | FAM/MGB-NFQ | ||
Metapneumovirus | F | AGCTTCAGTCAATTCAACAGAAG | ||
R | CCTGCAGATGTYGGCATGT | F | ||
P | TGTTGTGCGGCAGTTTTCAGACAATGC | LCRED670/BBQ | ||
Parechovirus | F | CTGGGGCCAAAAGCCA | ||
R | GGTACCTTCTGGGCATCCTTC | 5′-UTR | ||
P | AAACACTAGTTGTAWGGCCC | FAM/MGB-NFQ | ||
Parainfluenzavirus 1 | F | ATCTCATTATTACCYGGACCAAGTCTACT | ||
R | CATCCTTGAGTGATTAAGTTTGATGAATA | HN | ||
P | AGGATGTGTTAGAYTACCTTCATTATCAATTGGTGATG | CYAN500/DB | ||
Parainfluenzavirus 2 | F | CTGCAGCTATGAGTAATC | ||
R | TGATCGAGCATCTGGAAT | NP | ||
P | AGCCATGCATTCACCAGAAGCCAGC | LCRED610/BBQ | ||
Parainfluenzavirus 3 | F | ACTCTATCYACTCTCAGACC | ||
R | TGGGATCTCTGAGGATAC | NP | ||
P | AAGGGACCACGCGCTCCTTTCATC | LCRED670/BBQ | ||
Parainfluenzavirus 4 | F | GATCCACAGCAAAGATTCAC | ||
R | GCCTGTAAGGAAAGCAGAGA | NP | ||
P | TATCATCATCTGCCAAATCGGCAA | HEX/BHQ | ||
HBoV | F | CAAATCTCTTCTGGCTACACG | ||
R | CTCTGCGATCTCTATATTGAAGG | NS1 | ||
P | ATGTTGCCGCCAGTAACTCCACC | LCRED670/BBQ | ||
IC (EAV) | F | CATCTCTTGCTTTGCTCCTTAG | ||
R | AGCCGCACCTTCACATTG | Replicase | ||
P | TGTGGGCAATAATGTTGTTCTGACAGCG | HEX/BHQ |
+ denotes a locked nucleic acid (LNA) nucleotide.