Table 3.
Clinical evaluation.
| (A) Analysis of prospectively collected nasopharyngeal aspirates (n = 133) from children admitted to the Academical Medical Centre for an acute respiratory tract infection during the winter of 2007–08 | |||
|---|---|---|---|
| Single target PCR | Multiplex PCR | ||
| InfA | 9/133 | 9/133 | (p = 1.00a) |
| InfB | 3/133 | 3/133 | (p = 1.00b) |
| EV | 7/133 | 1/133 | (p = 0.01b) |
| ADV | 15/133 | 14/133 | (p = 0.86a) |
| RV | 36/133 | 36/133 | (p = 1.00a) |
| RSV | 23/133 | 22/133 | (p = 0.89a) |
| hMPV | 7/133 | 8/133 | (p = 0.80a) |
| PIV-1 | 2/133 | 2/133 | (p = 1.00b) |
| PIV-2 | 1/133 | 1/133 | (p = 1.00b) |
| PIV-3 | 1/133 | 2/133 | (p = 1.00b) |
| PIV-4 | 1/133 | 1/133 | (p = 1.00b) |
| hPeV | 4/133 | 3/133 | (p = 0.70b) |
| hCoV | 3/133 | 4/133 | (p = 0.70b) |
| hBoV | 8/133 | 7/133 | (p = 0.80a) |
| Total | |||
| Excluding EV | 113/133 | 112/133 | (p = 0.96a) |
| Including EV | 120/133 | 113/133 | (p = 0.73a) |
| (B) Quality control panel (2007) for PIV 1, 2, 3, 4, InfB, hPeV and hCoV (n = 10) | ||||
|---|---|---|---|---|
| Single target PCR | Multiplex PCR | QCMD | ||
| PIV-1 | 4/10 | 4/10 | 4/10 | (p = 1.00b) |
| PIV-2 | 1/10 | 1/10 | 1/10 | (p = 1.00b) |
| PIV-3 | 1/10 | 1/10 | 1/10 | (p = 1.00b) |
| PIV-4 | 3/10 | 3/10 | 3/10 | (p = 1.00b) |
| InfB | 1/14 | 1/14 | 1/14 | (p = 1.00b |
| hCoV | 5/14 | 5/14 | 5/14 | (p = 1.00a) |
Chi square
Fisher exact test.