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Summary of purification of dull black soybean (G. max) trypsin inhibitor from 100 g seeds
| Total protein (mg) | Total trypsin-inhibitory activity (U × 104) | Specific activity (U/mg) | Purification fold | Recovery of protein in mass (%) | |
|---|---|---|---|---|---|
| Crude Extract | 14583 | 551 | 378 | 1 | 100 |
| Q1 (After Q-Sepharose) | 4627 | 436 | 857 | 2.3 | 31.7 |
| SP1 (After SP-Sepharose) | 1166 | 314 | 2694 | 7.1 | 8.0 |
| S3 (After Mono Q) | 140 | 161 | 11500 | 30.5 | 0.9 |
| After Superdex 75 | 43 | 105 | 24419 | 64.6 | 0.3 |
