Table 2.
Comparison of individual RT-qPCR and TAC assay results.
| Virus | Characteristic | Individual RT-qPCR Assay | TAC assay | % Sensitivity (95% CI)a |
|---|---|---|---|---|
| Adenovirus | No. positive | 81 | 44 | 54 (43–65) |
| Mean CT ± SD | 30.74 ± 6.31 | 30.60 ± 5.17 | ||
| Median CT (Range) | 33.27 (15.03–38.27) | 31.10 (16.31–39.58) | ||
| HMPV | No. positive | 73 | 63 | 86 (76–93) |
| Mean CT ± SD | 28.03 ± 6.11 | 29.34 ± 5.09 | ||
| Median CT (Range) | 28.32 (13.04–39.44) | 29.03 (18.02–38.63) | ||
| HPIV-1 | No. positive | 16 | 9 | 56 (31–79) |
| Mean CT ± SD | 30.68 ± 4.84 | 35.88 ± 3.74 | ||
| Median CT (Range) | 30.00 (22.30–39.40) | 36.01 (29.63–40.00) | ||
| HPIV-2 | No. positive | 4 | 3 | 75 (22–99) |
| Mean CT ± SD | 32.14 ± 6.24 | 33.31 ± 5.36 | ||
| Median CT (Range) | 33.97 (23.15–37.48) | 36.10 (27.13–36.69) | ||
| HPIV-3 | No. positive | 44 | 36 | 82 (67–91) |
| Mean CT ± SD | 24.88 ± 7.34 | 25.30 ± 4.50 | ||
| Median CT (Range) | 24.07 (13.28–38.01) | 25.31 (18.24–34.82) | ||
| HPIV-4 | No. positive | 12 | 11 | 92 (60–99) |
| Mean CT ± SD | 32.57 ± 4.44 | 30.74 ± 4.34 | ||
| Median CT (Range) | 33.15 (24.93–39.47) | 32.04 (23.15–36.44) | ||
| Influenza A | No. positive | 115 | 109 | 95 (89–98) |
| Mean CT ± SD | 29.62 ± 5.22 | 27.77 ± 4.67 | ||
| Median CT (Range) | 29.28 (18.40–39.62) | 27.63 (18.23–36.47) | ||
| Influenza B | No. positive | 25 | 23 | 92 (72–99) |
| Mean CT ± SD | 30.38 ± 5.53 | 26.74 ± 4.43 | ||
| Median CT (Range) | 29.61 (22.09–39.64) | 27.22 (20.05–34.19) | ||
| Rhinovirus | No. positive | 271 | 218 | 80 (75–84) |
| Mean CT ± SD | 28.58 ± 5.40 | 30.23 ± 4.61 | ||
| Median CT (Range) | 28.60 (12.72–39.69) | 30.81 (17.47–40.00) | ||
| RSV | No. positive | 201 | 189 | 94 (90–97) |
| Mean CT ± SD | 23.65 ± 4.94 | 24.78 ± 4.09 | ||
| Median CT (Range) | 22.46 (14.75–39.36) | 24.09 (16.20–35.35) |
a
Sensitivity of TAC assay calculated assuming individual RT-qPCR test results as “gold standard”.