Fig. 3.

Peptide C6 exhibited characteristics of amyloid-like fibrils. a. Peptide C6 contained a predicted amyloid-like fibril-forming segment and exhibited β-strand content in an aqueous environment. The Cluster I consensus sequence is in bold and the predicted amyloid-like fibril-forming segment underlined. The CD spectrum of C6 (1 mM) in H₂O was shown with its estimated secondary structure contents. NRMSD, normalized root mean square deviation. b. The aged C6 exhibited maximal absorbance at 540 nm when mixed with Congo Red. c. The aged C6 had an increased fluorescence emission at 482 nm, when mixed with ThT.