Fig. 2.

A) Optimization of reaction temperature for ribavirin bioconversion. Biotransformation was performed during 1 h using 2.5 mM Urd and TCA in sodium phosphate buffer (30 mM, pH 7) at different temperatures and 200 rpm. According to Duncan test for data media comparison, significant differences among treatments named with different letter (p-value < 0.05) were observed. Productivity was calculated as: mmol ribavirin/L × h. B) Biotransformation was evaluated using different amounts of substrates, 10 mM TCA: 2.5 mM Urd (○); 2.5 mM TCA: 2.5 mM Urd (□); 2.5 mM TCA: 10 mM Urd (▴) at 30 °C and 200 rpm. All reactions were performed three times and bioconversion percentage was calculated as: (mmol product/mmol limiting reagent) × 100.