Skip to main content
. 2018 Apr 10;70:45–54. doi: 10.1016/j.procbio.2018.04.006

Fig. 6.

Fig. 6

Antigen-binding activity of crude extract from leaves of N. benthamiana harvested at 7 d.p.i. and purified GRFT from E. coli. To detect the specific antigen-binding activity of plant GRFT, plates were coated with 100 ng of HIV-1 gp120 protein, and bound of purified GRFT was detected with GRFT anti-rabbit primary antibody (1:1000 in PBST) and HRP anti-rabbit secondary antibody (1:2000 in PBST). NT-GRFT-450 mM NaCl = GRFT expressed by extraction buffer contained 450 mM NaCl; NT-GRFT = GRFT expressed by the pBIN6:GRFT cassette in N. benthamiana; C+ = GRFT expressed from E. coli as the positive control; WT = wild-type plants; C− = negative control (PBS). OD, optical density at 450 nm. Both purified NT-GRFT and NT- GRFT 450 mM NaCl showed higher binding activity compared with PBS as the negative control and WT. Values are the average of three experiments ± SD.