. 2007 Aug 21;40(1):9–14. doi: 10.1016/j.jcv.2007.07.015

Table 5.

KIV and WUV real-time PCR cycle threshold (Ct) values and conventional reference PCR results for serial dilutions of quantified KIV or WUV reference genomic DNA

	Estimated KIV genome copy number
	1.0E⁶	1.0E⁵	1.0E⁴	1.0E³	1.0E²	1.0E¹	1.0E⁰
KI-A	17.8^a	21.8^a	24.4^a	28.4^a	31.7^a	35.1^a	ND^b
KI-B	15.9^a	19.4^a	22.7^a	26.7^a	29.9^a	33.8^a	ND^c
Conventional	Pos	Pos	Pos	Pos	Pos	ND^b	ND

	Estimated WUV genome copy number
	1.0E⁵	1.0E⁴	1.0E³	1.0E²	1.0E¹	1.0E⁰
WU-A	19.9^a	23.9^a	27.9^a	31.9^a	35.8^a	ND
WU-B	20.0^a	23.4^a	26.8^a	31.6^a	35.7^a	ND
WU-C	17.9^a	22.2^a	26.1^a	30.0^a	33.7^a	ND
Conventional	Pos	Pos	Pos	Pos	ND	ND

Pos = positive. ND = not detected.

Mean Ct values of triplicate reactions.

Template detected in 2 of 3 replicates.

Template detected in 1 of 3 replicates.