Table 3.
References of real-time single target NAAT protocols
| Author | Targets | Procedure |
|---|---|---|
| Influenza virus | ||
| Habib-Bein et al. (2003) | Matrix IFLA | Smart Cycler |
| Henrickson (2004) | IFLA nucl. caps. gene | NASBA ECL |
| Parainfluenza | ||
| These viruses are generally part of multiplex reactions | ||
| RSV | ||
| Whiley et al. (2002) | L gene | Light Cycler |
| van Elden et al. (2003) | N gene types A, B | TaqMan |
| Mentel et al. (2003) | F gene | TaqMan |
| Gueudin et al. (2003) | N gene | Light Cycler |
| Borg et al. (2003) | F gene | TaqMan |
| Kuypers et al. (2004) | Matrix protein gene | TaqMan |
| O'Shea and Cane (2004) | N gene | AmpliTaq |
| Perkins et al. (2005) | F, N genes types A, B | AB Prism 7900HT |
| Human metapneumovirus | ||
| Cote et al. (2003) | N gene | Light Cycler |
| Mackay et al. (2003) | N gene | Light Cycler |
| Maertzdorf et al. (2004) | N gene | ABI Prism 7000 |
| Coronavirus | ||
| Drosten et al. (2003) | sars BNI fragment | Light Cycler/BioSystems 7000 SDS |
| Poon et al. (2003) | sars RNA polymerase | Light Cycler |
| van Elden et al. (2004) | (229E, OC43) N gene sars polymerase gene seminested | TaqMan ABI Prism 7700 |
| Fouchier et al. (2004) | (NL-63) N gene | TaqMan |
| Mahony et al. (2004) | sars, nuclocapsid gene | Light Cycler |
| van der Hoek et al. (2004) | NL-63 | i Cycler |
| Emery et al. (2004) | sars, polymerase, two regions of nucleocapsid | TaqMan, i Cycler |
| Hourfar et al. (2004) | sars Roche replicase “Aztus” (genome fragment) | Roche assays and Aztus |
| Nitsche et al. (2004) | sars, three different sequences | BioSystems DS 7700, 7000, Light Cycler |
| Booth et al. (2005) | sars, P, N, M genes | i Cycler |
| Woo et al. (2005) | HKU1 N gene | Light Cycler |
| Chui et al. (2005) | Comparison of nine primer seq. | |
| Adenovirus | ||
| Poddar (1999) | Hexon gene | TaqMan |
| Houng et al. (2002) | Hexon gene | TaqMan |
| Heim et al. (2003) | Hexon gene | Light Cycler |
| Rhinovirus | ||
| Nijhuis et al. (2002) | 5′-Non-coding region | N-A |
| Dagher et al. (2004) | 5′-NCR | Light Cycler |
| Kares et al. (2004) | 5′-NCR | Light Cycler |
| Bocavirus | ||
| Lu et al. (2006) | NS1, NP genes | Taqman |
| Mycoplasma pneumoniae | ||
| Ursi et al. (2003) | P1 | Light Cycler |
| Templeton et al. (2003a) | P1 | i Cycler |
| Loens et al. (2003b) | Review | |
| Chlamydophila pneumoniae | ||
| Mygind et al. (2001) | pmp 4 gene | Light Cycler |
| Kuoppa et al. (2002) | MOMP gene | ABI Prism 7700 |
| Tondella et al. (2002) | Two regions MOMP gene | AB Prisma 7700 |
| Ciervo et al. (2003) | Pst fragment | Light Cycler |
| Apfalter et al. (2003) | MOMP | TaqMan |
| Hardick et al. (2004) | 16S rRNA gene | TaqMan |
| Loens et al. (2006a) | 16S rRNA gene | NASBA |
| Legionella | ||
| Ballard et al. (2000) | mip gene L. pneumophila | Light Cycler |
| Wellinghausen et al. (2001) | 16S rRNA gene Leg. spp. | Light Cycler |
| Rantakokko-Jalava and Jalava (2001) | 16S rRNA gene Leg. spp. | Light Cycler |
| Hayden et al. (2001) | 5S rDNA Leg. genus mip Leg. spp. | Light Cycler |
| Wilson et al. (2003) | mip gene L. pneumophila | Light Cycler |
| Loens et al. (2006d) | 16 Rrna gene | NASBA |
For abbreviations see Table 2.