TABLE 1.
Primers used for quantitative RT-PCR
| Common name | Primer sequences (5′–3′) | Gene symbol |
|---|---|---|
| HPRT | ||
| Forward | CAGTACAGCCCCAAAATGGT | Hprt1 |
| Reverse | CAGTACAGCCCCAAAATGGT | |
| GAPDH | ||
| Forward | CGTCCCGTAGACAAAATGGT | Gapdh |
| Reverse | TCAATGAAGGGGTCGTTGAT | |
| β-Actin | ||
| Forward | GTCCACCTTCCAGCAGATGT | Actb |
| Reverse | AAGGGTGTAAAACGCAGCTC | |
| PMCA2 | ||
| Forward | AGAGAAGTCGGTGCTTCAGG | Atp2b2 |
| Reverse | GAAGGTGTCCACGGTGAAGT | |
| SERCA2 | ||
| Forward | CCCGAAACTACCTGGAACAA | Atp2a2 |
| Reverse | AGGGCTGGTAGATGTGTTGC | |
| SPCA1 | ||
| Forward | TCTCTGTGGGGGACAGAGTT | Atp2c1 |
| Reverse | GCTGAGGGGCTGTCACTTTA | |
| SPCA2 | ||
| Forward | GAGCGTCCTCACCAAAGAAT | Atp2c2 |
| Reverse | CCAGAGACTTCTCCGACCTG | |
| K18 | ||
| Forward | CTTGCGAATTCTGTGGACAA | Krt1–18 |
| Reverse | AGTCCATGGATGTCGCTCTC |