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. 2009 May 7;1(5-6):345–362. doi: 10.1039/b905502a

Fig1.

Fig1

Principle of an antibody microarray. (A) Probeantigens corresponding to different target antibodies are attached to a glass slide in an array format. (B) The slide is reacted with a solution containing target antibodies corresponding to two of the antigens. A fluorescent label antibody is then attached to the target antibody, so that the spots containing target become fluorescent, indicating binding. The spatial position of the fluorescent spot identifies the antigen and hence the target antibody.