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. 2009 May 7;1(5-6):345–362. doi: 10.1039/b905502a

Fig6.

Fig6

Process for binding and readout of NanoString barcodes. (A) Capture probes and reporter probes are reacted with a sample and bind to complementary RNA strands. (B) The biotinylated capture probes are immobilized on a streptavidin coated slide. The reporter probes are all aligned in the same direction by an electric field, and fixed to the slide via short biotinylated RNA strands. (C) Fluorescence microscope images are analyzed along the alignment direction to readout the codes (adapted by permission from Macmillan Publishing Ltd: Nature Biotechnology,36 copyright 2008).