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. 1999 Sep;9(9):897–906. doi: 10.1093/glycob/9.9.897

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Fig. 2. — Separation of N-linked oligosaccharides from C-CAM by anion exchange chromatography. N-glycans were fractionated according their number of negative charges using anion-exchange chromatography on MonoQ as described in Materials and methods. Fractions of neutral (A0), mono- (α1), bi- (α2), tri- (A3.1, A3.2), and tetrasialylated (A4) oligosaccharides were desialylated and further characterized using aminophase HPLC.