Fig. 5.
Separation of N-linked oligosaccharides from C-CAM by anion exchange chromatography before (A) or after digestion with sialidases from Newcastle disease virus (B) and Arthrobacter ureafaciens (C), respectively. N-glycans were fractionated according their number of negative charges using anion-exchange chromatography on MonoQ as described in Materials and methods. A0, Neutral; α1, mono-; α2, bi-; A3.1 and A3.2, tri- and A4 tetrasialylated structures.