Skip to main content
. 2009 Aug 14;21(10):1125–1134. doi: 10.1093/intimm/dxp078

Fig. 4.

Fig. 4.

mMBL-C-CRD protein blocks the adhesion of Shigella flexneri 2a to intestinal epithelial tissue. Frozen section of intestinal tissue was incubated with TRITC-labeled S. flexneri 2a (a, b and c) for 3 h. For antibody blocking study, the section was incubated with anti-CRD antibody for 1 h before the bacterial incubation (d, e and f). In another group, TRITC-labeled S. flexneri 2a was incubated with Trx-CRD for 1 h before adding to frozen sections (g, h and i). After being washed with TBS, fixed with 4% PFA and immunostained with FITC-labeled monoclonal anti-cytokeratin antibody, the slides were analyzed by fluorescence microscope and the amounts of adhesive bacteria were compared by microscopic count data from counting five fields on three slides for each group. The tissue slide treated with anti-CRD antibody displayed more adhesive bacteria, and the adhesive bacteria was significantly decreased when mMBL-C-CRD protein was added (**P < 0.01 versus control).