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. 2020 Mar 24;14:245. doi: 10.3389/fnins.2020.00245

FIGURE 6.

FIGURE 6

Effects of Nix on the neuronal apoptosis and degeneration at 24 h after SAH. (A,B) TUNEL staining showed apoptotic cells in the Sham, SAH, SAH + Vec, SAH + over-Nix, SAH + Ctr siRNA, and SAH + Nix siRNA groups. Double-immunofluorescent staining was performed with TUNEL (green) and a neuronal marker (NeuN, red), and nuclei were fluorescently labeled with DAPI (blue), Scale bar = 20 μm; **p < 0.01 vs. Sham group; NSp > 0.05 vs. SAH group; ##p < 0.01 vs. SAH + Vec group; &&p < 0.01 vs. SAH + Ctr siRNA group; n = 6. (C,D) FJB fluorescence staining (green) showing neuronal degeneration. Scale bar = 20 μm. **p < 0.01 vs. Sham group; NSp > 0.05 vs. SAH group; ##p < 0.01 vs. SAH + Vec group; &&p < 0.01 vs. SAH + Ctr siRNA group; n = 6.