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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: Dev Cell. 2020 Jan 2;52(3):309–320.e5. doi: 10.1016/j.devcel.2019.12.007

Figure 1. Long-range motion of the INO1 locus to the nuclear periphery is p23 and Hsp90 dependent.

Figure 1.

(A) Representative images of the GFP-marked INO1 locus, which is visualized using an integrated array of lac binding sites near INO1 that are bound by GFP-LacI, in the presence of inositol (+) and absence (−). The outer and nuclear membranes are marked by ER05-mCherry (Randise-Hinchliff et al., 2016). (B) The reliance on p23 for inositol-dependent movement of INO1 was checked using p23 null (p23Δ) and parental yeast cells. (C) The impact of Hsp90 on INO1 motion was tested in the absence (DMSO), presence of an Hsp90 inhibitor (Radicicol), or after removal of the drug (Withdrawal). All INO1 motion data represent averages of 3 independent experiments with at least 50 counted cells in each trial and the error bars represent the SEM.