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. 2020 Mar 31;15(3):e0229892. doi: 10.1371/journal.pone.0229892

Table 1. Primer sequences for RT-qPCR.

Gene Gene bank ID Primer sequences Size (bps)
NF-H NM_001003352.1 F: 5ʹ-GGAGGTTCCTGCCAAGGTGA-3ʹ 109
R: 5ʹ-CTCTGCTGCTTTGCTGGGTTC-3ʹ
Ascl1 XM_539745.3 F: 5ʹ-CAACTAAGCATTGCCTGCCTGA-3ʹ 154
R: 5ʹ-CCCAGTGCCTTTGCACACA-3ʹ
NeuroD2 XM_548146.3 F: 5ʹ-GGACTACAACAGCTCCGAGTACGA-3ʹ 133
R: 5ʹ-CAGCGCCGAGTAGTGCATAGAA-3ʹ
NES XM_547531.2 F: 5ʹ-GGACGGGCTTGGTGTCAATAG-3ʹ 91
R: 5ʹ-AGACTGCTGCAGCCCATTCA-3ʹ
GFAP XM_537614.2 F: 5ʹ-GCAGAAGTTCCAGGATGAAACCA-3ʹ 107
R: 5ʹ-TCTCCAGATCCAGACGGGCTA-3ʹ
NF-L XM_534572.2 F: 5ʹ-TGAATATCATGGGCAGAAGTGGAA-3ʹ 140
R: 5ʹ-GGTCAGGATTGCAGGCAACA-3ʹ
NGFR XM_548191.3 F: 5ʹ-ATGGGCAGCTCTCAGCCTGTA-3ʹ 105
R: 5ʹ-AATGTAGGCCACTAAGCCCACAAC-3ʹ
CHAT XM_543902.3 F: 5ʹ-GACACACTGGTGGCCCAGAA-3ʹ 107
R: 5ʹ-CGGCGGAAATTAATGACAACATC-3ʹ
TH NM_001002966.1 F: 5ʹ-AAGTTTGACCCTGACCTGGACTTG-3ʹ 100
R: 5ʹ-GCTTGTATTGGAATGCGATCTCTG-3ʹ
GLS XM_545570.5 F: 5ʹ-GGGCACCACGACGTGTTTAAG-3ʹ 124
R: 5ʹ-GCGGTTACAAGAGTCCGTCCA-3ʹ
GAD NM_001097543.1 F: 5ʹ-TGGAGCTGCACTTGGCTTTG-3ʹ 148
R: 5ʹ-GCCAGCAGTTGCATTGACGTA-3ʹ
TBP XM_863452 F: 5'-ACTGTTGGTGGGTCAGCACAAG-3' 124
    R: 5'-ATGGTGTGTACGGGAGCCAAG-3'