Figure 3.

Let-7a-5p is a direct target of circ-ABCB10 in BC cells. (A) Let-7a-5p was predicted as a target of circ-ABCB10 by Starbase. (B, C) Dual-luciferase reporter assay was conducted to verify the combination between circ-ABCB10 and let-7a-5p in MCF-7/PTX and MDA-MB-231/PTX cells. (D, E) The abundance of circ-ABCB10 and let-7a-5p was determined in MCF-7/PTX and MDA-MB-231/PTX cells transfected with si-NC, si-circ-ABCB10, pcDNA or circ-ABCB10 by qRT-PCR. MCF-7/PTX and MDA-MB-231/PTX cells were transfected with si-NC, si-circ-ABCB10, si-circ-ABCB10 + anti-miR-NC or si-circ-ABCB10 + anti-let-7a-5p. (F) QRT-PCR was conducted to measure the abundance of let-7a-5p in the above two cells. (G) MTT assay was applied to examine the IC₅₀ of PTX in the above two cells. (H) Flow cytometry was applied to analyze the apoptosis rate of the above MCF-7/PTX and MDA-MB-231/PTX cells. (I) The invasion of the above two cells was determined by transwell invasion assay. (J, K) The abundance of LC3-I, LC3-II and P62 was measured in MCF-7/PTX and MDA-MB-231/PTX cells by Western blot assay. *P<0.05.