Fig 1. CRISPR/Cas9-mediated knockout of HaABCC2 and HaABCC3.

A) Genomic DNA. C2-sgRNA1 and C2-sgRNA2 target exons 4 and 24 in HaABCC2. C3-sgRNA1 and C3-sgRNA2 target exons 4 and 25 in the adjacent gene, HaABCC3. Boxes show exons and lines show introns. B) Protein structure for both HaABCC2 and HaABCC3. The number 1 shows the target sites of C2-sgRNA1 and C3-sgRNA1 in transmembrane (TM) region III; 2 shows the target sites of C2-sgRNA2 and C3-sgRNA2 near the carboxy terminus. C) Sequences of the wild type (WT) and knockouts (C2-KO, C3-KO, and C2/3-KO) detected in G₁. Dashes show deleted bases and lower case letters show inserted bases. For HaABCC2, the same three knockout sequences occurred in C2-KO and C2/3-KO. For HaABCC3, one knockout sequence (large fragment missing) occurred in C3-KO and a different knockout sequence (two small fragments missing) occurred in C2/3-KO.