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. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969

Fig 8. The periplasmic domain of PhoQ is needed for the regulation of the T6SS.

Fig 8

(A, B) Expression of T6SS by Kp52145, 52145-ΔphoPQGB (ΔphoPQ), 52145-ΔphoQGBComFLAG (ΔphoPQ::phoPQFLAG), 52145-ΔphoQGBComPhoQ45-190 (ΔphoPQ::phoPQ45-190), and 52145-ΔphoQGBComPhoQGNNNNAQ (ΔphoPQ::phoPQGNNNNAQ) carrying the transcriptional fusion tssB::lucFF after incubation with K. pneumoniae ATCC43816 (A) and A. baumannii ATCC17978 (B). Luminescence is expressed as relative light units (RLU) pero CFU (x109). #, results are significantly different (P < 0.0001) for the indictaed comparisons; n.s., not significant (P > 0.05) from the results for mock-treated Kp52145 (mock); one-way ANOVA Bonferroni correction for multiple comparisons. (C, D) T6SS-dependent anti-bacterial activity as determined by recovery of target organisms K. pneumoniae ATCC43816 (Kp43816) (C), A. baumannii ATCC17978 (17978) (D) following incubation with Kp52145, 52145-ΔphoPQGB (ΔphoPQ), 52145-ΔphoQGBComFLAG (ΔphoPQ::phoPQFLAG), 52145-ΔphoQGBComPhoQ45-190 (ΔphoPQ::phoPQ45-190), and 52145-ΔphoQGBComPhoQGNNNNAQ (ΔphoPQ::phoPQGNNNNAQ). In all panels, #, P < 0.0001, n.s. (P > 0.05), not significant differences from the results for PBS-treated (mock) target cell; one-way ANOVA Bonferroni correction for multiple comparisons. In all panels, the data are presented as means ± the standard deviations (n = 3).