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. 2020 Mar 19;16(3):e1007969. doi: 10.1371/journal.ppat.1007969

Fig 10. K. pneumoniae T6SS-dependent antifungal competition.

Fig 10

(A, B) T6SS-dependent anti-bacterial activity as determined by recovery of target organism C. albicans, and S. cerevisiae following incubation with Kp52145, 52145-ΔclpV (ΔclpV), 52145-ΔclpV complemented with pBADClpV (ΔclpV/pBADClpV), 52145-ΔvgrG1 (ΔvgrG1), 52145-ΔvgrG1 harbouring pBADVgrG1 (ΔvgrG1/ pBADVgrG1), 52145-ΔvgrG2 (ΔvgrG2), 52145-ΔvgrG2 harbouring pBADVgrG2 (ΔvgrG2/ pBADVgrG2), 52145-ΔvgrG4 (ΔvgrG4), 52145-ΔvgrG4 harbouring pBADVgrG4 (ΔvgrG4/ pBADVgrG4). After 6 h incubation in LB, target cells were recovered, diluted in PBS 0 to 10−6 and spotted in YNB agar plate. Mock, PBS-treated C. albicans and S. cerevisiae. In all panels, #, P < 0.0001, n.s. (P > 0.05), not significant differences from the results for PBS-treated (mock) target cell; one-way ANOVA Bonferroni correction for multiple comparisons. The data are presented as means ± the standard deviations (n = 3).