Table 3.
Deconvolution of Plasma GH Blood Profiles in WT and Igsf1∆312 Mice
| Mice | P Valuea | ||||||
|---|---|---|---|---|---|---|---|
| WT Aged 6 Wk | WT Aged 9 Wk | Igsf1 ∆312 Aged 6 Wk | Igsf1 ∆312 Aged 9 Wk | Between Groups | Time | Time × Group | |
| Pulse frequency (no./6 h) | 4.6 ± 0.4 | 4.3 ± 0.3 | 4.7 ± 0.5 | 3.6 ± 0.4 | 0.58 | 0.048 | 0.23 |
| Mode (min) | 15.3 ± 1.0 | 16.3 ± 0.6 | 14.8 ± 0.9 | 15.2 ± 0.8 | 0.30 | 0.46 | 0.76 |
| Slow half-life (min) | 6.4 ± 0.6 | 6.2 ± 0.7 | 7.2 ± 0.6 | 7.1 ± 0.6 | 0.18 | 0.81 | 0.99 |
| Basal secretion (ng/mL/6 h) | 222 ± 127 | 313 ± 104 | 110 ± 28 | 171 ± 28 | 0.72 | 0.029 | 0.86 |
| Pulsatile secretion (ng/mL/6 h) | 539 ± 118 | 721 ± 148 | 801 ± 166 | 652 ± 140 | 0.52 | 0.96 | 0.27 |
| Total secretion (ng/mL/6 h) | 762 ± 226 | 1034 ± 219 | 912 ± 185 | 823 ± 157 | 0.62 | 0.62 | 0.32 |
| Mean pulse mass (ng/mL) | 132 ± 33 | 179 ± 35 | 186 ± 36 | 235 ± 60 | 0.37 | 0.33 | 0.68 |
| ApEn (dimensionless) | 0.791 ± 0.067 | 0.748 ± 0.069 | 0.804 ± 0.074 | 0.649 ± 0.067 | 0.61 | 0.056 | 0.28 |
Deconvolution analysis of plasma GH secretion profiles in male Igsf1Δexon1 and WT mice. Calculations were performed with an automated Matlab deconvolution program (34). Data are shown as mean ± SEM.
Abbreviations: ApEn, approximate entropy; WT, wild-type.
aData were analyzed with the 2-way ANOVA for repeated measures, assessing differences between WT and Igsf1Δ312 mice (between groups), differences between age 6 and 9 weeks (time), and the interaction between the effects of group and time (time × group). Where required, data were logarithmically transformed. The data at 6 and 9 weeks are from the same animals.