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. Author manuscript; available in PMC: 2020 Mar 31.
Published in final edited form as: Curr Protoc Mouse Biol. 2016 Dec 1;6(4):380–407. doi: 10.1002/cpmo.18

Figure 18.

Figure 18

Protocol for conditioned taste aversion using the modified two-bottle preference test. Step 1: Train water-restricted animals to reliably drink within allotted time. Continue until animals reliably drink 3 ml in 30 min. Step 2: Pair exposure to high concentration of tastant with malaise (by injection of LiCl) to generate a conditioned aversion to the tastant. Step 3: Confirm that animals acquired conditioned aversion. Step 4: Measure tastant perception threshold (concentration at which LiCl-injected animals fail to avoid the tastant). Step 5: Verify that animals still show a conditioned aversion to the high tastant concentration. Illustrations modified from Servier Medical Art (http://www.servier.com/Powerpoint-image-bank).