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. Author manuscript; available in PMC: 2020 Mar 31.
Published in final edited form as: Nat Metab. 2019 Jul 12;1(7):731–742. doi: 10.1038/s42255-019-0083-2

Figure 7. Alternative substrate utilization in LPS and IFN-γ stimulated macrophages.

Figure 7.

a. Fraction of citrate that remains unlabeled in RAW 264.7 cells stimulated with LPS and IFN-γ for indicated time, after 24h incubation with both U-13C-glucose and U-13C15N-glutamine. Bar graph with error bar represents mean + SD (n=3 independent samples), dots represent individual values.

b. Fraction of 2-labeled acetyl-CoA in RAW 264.7 cells stimulated with LPS and IFN-γ for indicated time, after 24h incubation with 40μM BSA-conjugated U-13C-palmitic acid. Bar graph with error bar represents mean + SD (n=3 independent samples), dots represent individual values.

c,d. Fraction of 2-labeled acetyl-CoA (c) and citrate (d) in RAW 264.7 cells stimulated with LPS and IFN-γ for indicated time, after 24h incubation with 500μM U-13C-acetate. Bar graph with error bar represents mean + SD (n=3 independent samples), dots represent individual values.