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. 2020 Mar 30;40(8):e00471-19. doi: 10.1128/MCB.00471-19

FIG 3.

FIG 3

IFN-β induced Ipr1 in RAW264.7 and J774A.1 cells. (A) IFN-β induced Ipr1 transcription in RAW264.7 cells. RAW264.7 cells were treated with TNF-α, IFN-β, and IL-13 for 8 h. The expression of Ipr1 was measured by qRT-PCR analysis (NC, sterile PBS containing 0.1% bovine serum albumin). The values of the treated groups were normalized to the mean of the NC groups (+SD). The statistical significance of differences in relative mRNA expression was assessed by two-way ANOVA (**, P < 0.01). (B) IFN-β induced Ipr1 transcription in J774A.1 cells. J774A.1 cells were treated with TNF-α, IFN-β, and IL-13 for 8 h, and the expression of Ipr1 was measured by qRT-PCR analysis. The values of the treated groups were normalized to the mean of the NC groups (+SD). The statistical significance of differences in relative mRNA expression was assessed by two-way ANOVA (**, P < 0.01). (C) Locus-specific mutation of STATx, Rel, and ISRE motifs in the Ipr1 promoter. The motifs were downloaded from JASPAR, and nucleotide mutation relied on conservation of specific sites.