FIG 1.

Immunoblot analysis of gH/gL complexes in parental and heterologous gO recombinant HCMV. Equal number of cell-free virions (as determined by qPCR) of HCMV TR (A), ME (B), or MT (C) or the corresponding heterologous gO recombinants were separated by reducing (upper two panes) or nonreducing (bottom pane) SDS-PAGE and analyzed by immunoblotting with antibodies specific for major capsid protein (MCP) or gL. Blots shown are representative of three independent experiments. Molecular mass markers (in kilodaltons) are indicated on each panel.