FIG 9.

Neutralization of parental HCMV TR and heterologous gO recombinant by anti-gH antibodies. Genome equivalents of extracellular HCMV TR or the corresponding heterologous gO recombinants were incubated with 0.025 to 250 μg/ml of anti-gH MAb 14-4b or 0.01 to 100 μg/ml of anti-gH MAb AP86 and then plated on cultures of nHDF (A and B) or ARPE19 epithelial cells (C and D). At 2 days postinfection, the number of infected (GFP⁺) cells was determined by flow cytometry and plotted as the percentage of the no-antibody control. (Left graphs) Full titration curves shown are representative of results from three independent experiments, each performed in triplicate. (Right graphs) Average percent of cells infected at the highest antibody concentrations in 3 independent experiments. Error bars represent SD. Asterisks indicate P values of ≤0.05, determined by one-way ANOVA with Dunnett’s multiple-comparison test comparing each recombinant to the parent.