TABLE 2.

Binding of parental and heterologous gO recombinant HCMV to fibroblasts

Virus	Expt 1a			Expt 2b			Expt 3c
	Meand	Folde	ANOVAf	Mean	Fold	ANOVA	Mean	Fold	ANOVA
TR_TR(GT1b)	17.8	—	—	31.2	—	—	30.4	—	—
TR_MEgO(GT5)	21.2	—	NS	44.7	1.4	*	37.9	—	NS
TR_PHgO(GT2a)	24.3	—	NS	12.7	0.41	*	35.3	—	NS
TR_TBgO(GT1c)	18.8	—	NS	30.5	—	NS	33.7	—	NS
TR_ADgO(GT1a)	25.7	—	NS	24.7	—	NS	23.3	—	NS
TR_TNgO(GT4)g	4.9	0.27	*	6.9	0.22	*	7.3	0.24	*
ME_MEgO(GT5)	21.6	—	—	5.8	—	—	7	—	—
ME_TR(GT1b)	5.3	0.25	*	7.1	—	NS	3.9	0.56	*
ME_PHgO(GT2a)	8.0	0.37	*	7.5	—	NS	2.3	0.33	*
ME_TBgO(GT1c)	15.9	0.74	*	9.0	—	NS	7	—	NS
ME_ADgO(GT1a)	2.4	0.11	*	2.4	—	NS	3.7	0.53	*
ME_TNgO(GT4)	5.8	0.27	*	8.5	—	NS	7.4	—	NS
MT_MEgO(GT5)	27.5	—	—	63.9	—	—	120.9	—	—
MT_TR(GT1b)	28.5	—	NS	40.2	0.63	*	159.4	—	NS
MT_PHgO(GT2a)	33.4	—	NS	50.4	—	NS	222	1.84	*
MT_TBgO(GT1c)	44.6	1.6	*	66.2	—	NS	220.8	1.83	*
MT_ADgO(GT1a)	8.5	0.31	*	13.4	0.21	*	23.6	0.2	*
MT_TNgO(GT4)	32.5	—	NS	61.8	—	NS	133.2	—	NS

^aInput concentration of cell-free virus stock (genomes/ml): TR-based viruses, 6.2 × 10⁷; ME-based viruses, 2.0 × 10⁸; MT-based viruses, 1.0 × 10⁸.

^bInput concentration of cell-free virus stock (genomes/ml): TR-based viruses, 7.5 × 10⁷; ME-based viruses, 5.0 × 10⁸; MT-based viruses, 2.0 × 10⁸.

^cInput concentration of cell-free virus stock (genomes/ml): TR-based viruses, 1.0 × 10⁸; ME-based viruses, 5.0 × 10⁸; MT-based viruses, 5.0 × 10⁸.

^dAverage pp150 puncta detected by immunofluorescence per cell in 10 microscopy fields; approximately 4 to 6 cells per field.

^eFold difference in mean pp150 puncta per cell compared to parental virus, determined for recombinant viruses that were significantly different (P ≤ 0.05) from parental within an experiment. —, value not calculated.

^fOne-way ANOVA with Dunnett’s multiple-comparison test comparing each recombinant to the parent. *, P ≤ 0.05. NS, not significant.

^gBold font indicates recombinant viruses that were significantly different from the parent in the same direction (> or <) in all 3 experiments.