FIG 7.

Neuroactive ligand-binding receptors counteract KSHV-infected endothelial cell proliferation. (A) GO analysis of neuroactive ligand-receptor interaction pathways in KSHV-infected cells. (B) Bar graph showing the neuroactive ligand-receptor pathways and the number of genes corresponding to each receptor pathway. (C) L1T2 cells were transduced with CRISPR-Cas9 HRH1 and CRISPR-Cas9 SSTR1 plasmids. Knockout (KO) cells were selected using puromycin hydrochloride, and the efficiencies of HRH1 and SSTR1 knockout were measured by Western blotting. β-Actin was used as a loading control. (D) To determine the effect of HRH1 and SSTR1 KO on cell proliferation, KO L1T2 cells were cultured for 48 h and pulsed with BrdU for 2 h, and BrdU incorporation was measured by using a BrdU cell proliferation ELISA kit. Error bars indicate the means ± SD of data from three experiments. *, P < 0.01; **, P < 0.001 (compared to the control).