TABLE 2.
Primers used for conventional RT-PCR and real-time qRT-PCR
| Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
| Conventional RT-PCR | ||
| ADM2 | GGAGCCTAAACACCCTGAAA-HairpinBlastHairpin | TGCATGAGAGCAGGAACAG |
| HRH1 | CCTCTGCTGGATCCCTTATTTC-HairpinBlast | CCAGGTCTGATTCTCTCTCTCT |
| NSE | CAGGACTTTGTCAGGGACTATC | GTAATCCCAGTGTGCTGTGA |
| PGP9.5 | CTGGGATTTGAGGATGGATCAG | TAATGCTCTGTGGGAGGGA |
| SSTR1 | GGAGGAGCCGGTTGACTATT | AAGGTAGCCTGAAAGCCTTCC |
| β-Actin | GCTCACCATGGATGATGATATCGCC | GGATGCCTCTCTTGCTCTGGGCCTC |
| Real-time qRT-PCR | ||
| ADM2 | CTGAGCCCCATCTGAAGCC | CAGCACTGCGTGTAGACCAG |
| HRH1 | GCACAGCGTCCATTTTCAGTG | TCGGGTCTTGGTACGATACTT |
| NSE/ENO2 | AGGTGCAGAGGTCTACCATAC | AGCTCCAAGGCTTCACTGTTC |
| UCHL1 | AATGTCGGGTAGATGACAAGGT | GGCATTCGTCCATCAAGTTCATA |
| SSTR1 | CCAGCATCTACTGTCTGACTGT | ATGACGAGCAGCGATAGCAC |
| CHGA | TAAAGGGGATACCGAGGTGATG | TCGGAGTGTCTCAAAACATTCC |
| SYP | CTCAGCATCGAGGTCGAGTTC | GAGGAGTAGTCCCCAACTAAGAA |