FIG 1.

Subcellular distributions of H129 viral particles in cultured cortical neurons. (A) Primary mouse embryonic cortical neurons were cultured in a microfluidic chamber for 14 days and then infected with H129-G/R at the soma side at an MOI of 1. Confocal images were captured at the indicated time points postinfection. (B) Primary mouse embryonic cortical neurons were cultured in 35-mm dish for 14 days and then were infected with H129-G4 at an MOI of 1. At 24 hours postinfection (hpi), neurons were fixed with 2.5% glutaraldehyde and collected for transmission electron microscopy (EM) analysis. Total viral particles, including capsids, enveloped virions, or other forms of viral particles were observed and analyzed. Capsids in the nuclei: empty A capsid; scaffold-containing B capsid; viral DNA-containing C capsid. (C) Viral particles in the cytoplasm: cytosolic A capsid (C1); cytosolic C capsid (C2); wrapping intermediate: capsids closely associated with cytoplasmic membrane (enveloping viral particles) (C3); complete virion after secondary envelopment (C4); complete virion with an extra membrane (C5); a complete virion along with other cargos wrapped within an extra vesicular structure (virions in MVB-like structure) (C6). (D) Viral particles in the neurites: neurite empty A capsid (d1); a neurite C capsid (d2); virions (d3); noninfectious particles (d4).