FIG 10.

Levels of type I IFN signaling proteins in cells infected with RRV strains, recombinants, and mutants. L929 cells were infected at an MOI of 2 with RRV-T48, RRV 2548, RRV M12, RRV M34, and RRV-T48 harboring the changes indicated above the panels. Cell were collected at 24 h p.i., lysed, and analyzed by Western blotting using antibodies recognizing RIG-I, MDA5, IPS-1, TRAF3, TBK1, IKK-i, IRF3, p-IRF3, IRF7, STAT1, STAT2, p-STAT1, and p-STAT2. Antibody against β-actin was used to detect loading control. Blots represent one of two independent reproducible experiments.