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. 2020 Feb 12;9:e54670. doi: 10.7554/eLife.54670

Figure 4. CBX action requires residues from both ECL1 and ECL2.

Figure 4.

(a and b) Surface (a) and cartoon (b) representations of the frPanx1 ECL1 (light blue) and ECL2 (dark blue), with potential CBX-interacting residues shown in orange. (c) Quantification of whole-cell currents from hPanx1 mutants when treated with CBX (100 μM). Mutants are numbered according to the hPanx1 sequence while the mutants in parenthesis are the corresponding residues in frPanx1. Recordings were performed by stepping to +100 mV in the absence or presence of CBX, and each point represents the normalized current amplitude during the CBX application. Bars represent the mean value from each mutant. Asterisks indicate significance of p<0.05 determined by one-way ANOVA followed by Dunnett’s test comparing WT to each mutant (F262C: p=0.0007; I247C: p=0.0471; V258C: p=0.0363).