Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Xenopus tropicalis) | frPanx1 | Synthesized by Genscript | NCBI Reference Sequence: NP_001123728.1 | Frog pannexin-1 gene sequence |
| Gene (Homo sapiens) | hPanx1 | Synthesized by Genscript | NCBI Reference Sequence: NP_056183.2 | Human pannexin-1 gene sequence |
| Cell line (Homo sapiens) | HEK293T cells | ATCC | Cat#: CRL-3216, RRID: CVCL_0045 | |
| Cell line (Spodoptera frugiperda) | Sf9 cells | ATCC | Cat#: CRL-1711, RRID: CVCL_0549 | |
| Recombinant DNA reagent | pIE2 hPanx1 | doi: 10.1085/jgp.201711804 | Mammalian expression vector for electrophysiology presented in Figure 1—figure supplement 1 and 2 | |
| Recombinant DNA reagent | pIE2 hPanx1 +GS | doi: 10.1085/jgp.201711804 | Mammalian expression vector for electrophysiology presented in Figures 1, 3 and 4 | |
| Recombinant DNA reagent | pIE2 frPanx1 | This paper | Mammalian expression vector for electrophysiology presented in Figure 1—figure supplement 1 and 2 | |
| Recombinant DNA reagent | pIE2 frPanx1 +GS | This paper | Mammalian expression vector for electrophysiology presented in Figure 1 | |
| Recombinant DNA reagent | pIE2 frPanx1-ΔLC | This paper | Mammalian expression vector for electrophysiology presented in Figure 1—figure supplement 1 and 2 | |
| Recombinant DNA reagent | pIE2 frPanx1-ΔLC +GS | This paper | Mammalian expression vector for electrophysiology presented in Figure 1 | |
| Recombinant DNA reagent | pC-NG-FB7 frPanx1-ΔLC | This paper | Insect cell/baculovirus expression construct | |
| Recombinant DNA reagent | pC-NG-FB7 frPanx1 | This paper | Insect cell/baculovirus expression construct | |
| Recombinant DNA reagent | pC-NG-FB7 hPanx1 | This paper | Insect cell/baculovirus expression construct | |
| Peptide, recombinant protein | MSP2N2 | doi: 10.1016/S0076-6879(09)64011–8 | nanodisc expression construct | |
| Commercial assay or kit | Fugene 6 | Promega | Cat#: E2691 | |
| Chemical compound, drug | Carbenoxolone | Sigma | Cat#: C4790 | |
| Chemical compound, drug | C12E8 | Anatrace | Cat#: APO128 | |
| Chemical compound, drug | DDM | Anatrace | Cat#: D310 | |
| Chemical compound, drug | Soybean polar lipid extract | Avanti | Cat#: 541602 | |
| Software, algorithm | cisTEM | doi: 10.7554/eLife.35383 | RRID: SCR_016502 | |
| Software, algorithm | Warp | doi: 10.1038/s41592-019-0580-y | ||
| Software, algorithm | Coot | doi: 10.1107/S0907444904019158 | RRID: SCR_014222 | |
| Software, algorithm | PHENIX | doi: 10.1107/S09074449052925 | RRID: SCR_014224 | |
| Software, algorithm | Axon pClamp 10.5 | Axon (Molecular Devices) | RRID: SCR_011323 |