Table 1. Cryo-EM data collection, refinement and validation statistics.
| frPanx- ΔLC (EMD-21150) (PDB: 6VD7) |
|
|---|---|
| Data collection and processing | |
| Magnification | 130,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 57.2 |
| Defocus range (μm) | 1.2–2.8 |
| Pixel size (Å) | 1.07 |
| Symmetry imposed | C7 |
| Initial particle images (no.) | 297374 |
| Final particle images (no.) | 90185 |
| Map resolution (Å) FSC threshold |
3.02 0.143 |
| Refinement | |
| Initial model used (PDB code) | de novo |
| Model resolution (Å) FSC threshold |
3.29 0.5 |
| Model resolution range (Å) | 3–6 |
| Map sharpening B factor (Å2) | −90 |
| Model composition Non-hydrogen atoms Protein residues Ligands |
16506 2079 0 |
| CC map vs. model (%) | 0.85 |
| R.m.s. deviations Bond lengths (Å) Bond angles (°) |
0.008 0.759 |
| Validation MolProbity score Clashscore Poor rotamers (%) |
1.92 5.96 0.78 |
| Ramachandran plot Favored (%) Allowed (%) Disallowed (%) |
88.32 11.68 0 |